¡Recombinant monoclonal antibodies
Part 8 – Final!
Recombinant DNA technology
We already know that monoclonal antibodies ( mAbs, or Moabs) are generated by immunizing animals with immunogens to provoke immune responses. B cells are then isolated from these immunized animals and fused with a myeloma cell line to create immortalized hybridomas, in the most classic form of production.
The technology used in recombinant monoclonal antibodies ( rAbs) is very different. rAbs are antibodies that are produced using recombinant DNA technology. Recombinant DNA technology involves the use of enzymes and various laboratory techniques to manipulate and isolate the DNA segments of interest. This method can be used to combine (or splice) DNA from different species or to create genes with new functions.
In simple terms, rAbs are generated in vitro using synthetic genes (outside the immune system). Therefore, no hybridomas or animals are needed in their production process.
a) The highest level of consistency and unmatched reproducibility,
b) Improved sensitivity and confirmed specificity,
c) High affinity, consistent between different batches,
d) Ease of scalability and continuity of supply,
e) High throughput in vitro manufacturing.
